原装现货|SubcellularProteinFractionationKit/亚细胞蛋白分离试剂盒

产品编号:78840   查看说明书产品名称:原装现货|SubcellularProteinFractionationKit/亚细胞蛋白分离试剂盒  .0   订购此产品 供应商:Pierce规格:kit目录价:5402库存状态:现货CAS编号: 应用范围: 种属来源: 相关信息: SubcellularProteinFractionationKit    Segregateandenrichproteinsfromfivecellularcompartments. TheThermoScientificSubcellularProteinFractionationKitenablessegregationandenrichmentofproteinsfromfivedifferentcellularcompartments. Thekitenablesthestepwiseseparationandextractionofcytoplasmic,membrane,nuclearsoluble,chromatin-boundandcytoskeletalproteinsfrommammalianculturedcells.ExtractsobtainedwiththeSubcellularProteinFractionationKitarecompatIBLewithavarietyofdownstreamapplicationsincludingWesternblotting,proteinassays,electrophoreticmobilityshiftassaysandreporter-geneandenzyme-activityassays.Fractionationofsubcellularproteinsenablesproteinlocalizationassessmentandproteinenrichmentfromspecificcellularcompartments.TheThermoScientificSubcellularProteinFractionationKitincludesacombinationofreagentsforstepwiselysisofcellsintofunctionalcytoplasmic,membrane,nuclearsoluble,chromatin-boundandcytoskeletalproteinfractionsinlessthan3hours.Extractsfromeachsubcellularcompartmentgenerallyhavelessthan15%contaminationbetweenfractions,whichissufficientpurityformostexperimentsstudyingproteinlocalizationandredistribution.HighlightsoftheSubcellularFractionationKits:Concise–extractfunctionalcytoplasmic,membrane,nuclearsoluble,chromatin-boundandcytoskeletalproteinfractionsinlessthan3hours Convenient–performasimpleprocedurewithoutusingultracentrifugationovergrADIents Complete–obtaincytoplasmic,membrane,solublenuclear,chromatin-boundandcytoskeletalproteinfractionsfromasinglekit Compatible–useextractsfordownstreamapplicationssuchasproteinassays,Westernblotting,gel-shiftassaysandenzymeactivityassays Applicationsforsubcellularfractionation:Extractandenrichproteinsfromdifferentcellularcompartments Isolatecytoplasmic,nuclear,membraneandcytoskeletalproteinsfromthesamecells Profilecellsforproteinsfromthecytoplasm,nucleus,membraneandCytoskeleton Studyproteintranslocation ProductDetails:TheSubcellularProteinFractionationKitcontainsfourextractionbuffers,astABIlizednucleaseandThermoScientificHaltProteaseInhibitorCocktail.Eachkithasenoughreagentstofractionate50cellpelletsof20µLeach,equivalenttoapproximately2gofcellpaste.Thefirstreagentaddedtoacellpelletcausesselectivemembranepermeabilization,releasingsolublecytoplasmiccontents.Thesecondreagentdissolvesplasma,mitochondriaandER-golgimembranesbutdoesnotsolubilizethenuclearmembranes.Afterrecoveringintactnucleibycentrifugation,athirdreagentyieldsthesolublenuclearextract.Anadditionalnuclearextractionwithmicrococcalnucleaseisperformedtoreleasechromatin-boundnuclearproteins.Therecoveredinsolublepelletisthenextractedwiththefinalreagenttoisolatecytoskeletalproteins.SubcellularProteinFractionationKit Formulation:Multi-componentkitSufficientFor:50sampleseachcontaining2millioncells(20µLpacked) KitContents: CytoplasmicExtractionBuffer,10mL MembraneExtractionBuffer,10mL NuclearExtractionBuffer,10mL PelletExtractionBuffer,5mL MicrococcalNuclease,150µL CalciumChloride,100mM,250µL HaltProteaseInhibitorCocktail(100X),350µL保存条件:按原厂要求保存